Glickman Michael H., Professor

Position:  Vice Dean
Phone:  +972-4-8294552

Building/Auditory:  438




"Mechanistic Studies of Soybean Lipoxygenase", University of California at Berkeley, 12/1994, Advisor: Professor Judith P. Klinman.


REFEREED PAPERS (original work)

1. Glickman M.H., Wiseman J., Klinman J.P., Extremely Large Isotope Effects in the Lipoxygenase-Linoleic acid Reaction, J. Am. Chem. Soc., 1994 (116), 793-794.


2. Glickman M.H., Klinman J.P., The Nature of the Rate Limiting Steps in the Soybean-Lipoxygenase-1 Catalyzed Oxidation of Linoleic Acid. Biochemistry, 1995 (34), 14077-14092


3. Glickman M.H., Klinman J.P., Lipoxygenase Reaction Mechanism - Demonstration that Hydrogen Abstraction from Substrate Precedes Dioxygen Binding during Catalytic Turnover, Biochemistry, 1996 (35), 12882-12892.


4. Jonsson T.#, Glickman M.H.#, Sun S.J.#, Klinman J.P., (#equal contributors) Experimental Evidence for Extensive Tunneling of the Heavier Isotopes of Hydrogen in Enzyme Reactions. J. Am. Chem. Soc., 1996 (118), 10319-10320.


5. Glickman M.H., Cliff S., Thiemens M.H., Klinman J.P., 17O and 18O isotope effects in the Soybean Lipoxygenase-1 Reaction; Implications for dioxygen activation., J. Am. Chem. Soc. 1997 (119), 11359-11261.


6. van Nocker S., Sadis S., Rubin D.M., Glickman M., Fu, H.Y., Coux O., Wefes I., Finley D., Vierstra R.D., The Multi-ubiquitin Chain Binding Protein Mcb1, is a component of the 26S in S. cerevisiae, and Plays a Nonessential Substrate Specific Role in Protein Turnover, Mol. Cell. Biol., 1996 (16) 6020-6028.


7. Rubin D.M., van Nocker S., Glickman M., Coux, O., Wefes I., Sadis S., Fu H., Goldberg A.L., Vierstra R., Finley D., ATPase and Ubiquitin-Binding Proteins of the Yeast Proteasome., Mol. Biol. Rep., 1997 (24) 17-26.


8. Moiseyev N.*, Rucker J., Glickman M.H.* (*Corresponding authors), Reduction of Ferric Iron could serve as an Electron Sink to drive Hydrogen Tunneling in the Lipoxygenase Reaction, J. Am. Chem. Soc. 1997 (119) 3853-3860.


9. Fu H., Sadis S., Rubin D.M., Glickman M.H., van Nocker S., Finley D., Vierstra, R.D., Multiubiquitin Chain Binding and Protein Degradation are Mediated by Distinct Domains within the 26S Proteasome Subunit. MCB1, J. Biol. Chem. 1998 (273) 1970-1989.


10. Glickman M.H., Rubin D.M., Fried V., Finley D., The Regulatory Particle of the S. cerevisiae proteasome. Mol. Cell. Biol. 1998 (18) 3149-3162.


11. Rubin D.M., Glickman M.H., Finley D. Multiple roles for ATP in the proteasome revealed by active site mutants in the six regulatory particle ATPases. EMBO J. 1998 (17), 4909-4919.


12. Glickman M.H., Rubin D.M., Fried V., Coux O., Fu H.Y., Vierstra R.D., Finley D. A subcomplex of the regulatory particle of the proteasome necessary for ubiquitin-conjugate degradation and related to the COP9/Signalosome and eIF3. Cell 1998 (94) 615-623.


13. Glickman M.H., Rubin D.M., Fu H., Larsen C.N., Coux O., Pfeifer G., Vierstra R., Baumeister W., Fried V., Finley D. Functional Analysis of the proteasome regulatory particle. Mol. Biol. Rep., 1999 (26) 21-28


14. Beate B., Glickman M.H., Kloetzel P.M., Finley D., Schmidt M., The base of the regulatory particle of the proteasome can function as a molecular chaperone. Nature Cell Biology 1999 (1) 221-226.


15. Groll M*, Bajorek M., Kohler A., Huber R., Glickman M.H.*, Finley D.* (*Corresponding authors), A gated channel into the proteasome, Nature Struct. Biol. 2000 (7) 1062-1067.


16. Köhler A., Bajorek M., Groll M., Moroder L., Rubin D., Huber R., Glickman M.H., Finley D., The substrate translocation channel of the proteasome, Biochemie 2001 (3-4) 325-332.


17. Fu H.Y.*, Reis, N., Lee, Y., Glickman, M.H.*, Vierstra, R.* (*Corresponding authors), Subunit interaction maps for the regulatory particle of the 26s proteasome and the cop9 signalosome reveal a conserved core structure, EMBO J. 2001 (20) 7096-7107


18. Maytal-Kivity V., Reis N., Hofmann K., Glickman M.H., MPN+, a putative catalytic motif common to eukaryotic and prokaryotic proteins, is critical for Rpn11 function, BMC Biochemistry, 2002 3:28


19. Maytal-Kivity V., Piran, R. Pick E., Hofmann K., Glickman M.H., COP9-signalosome complex components play a role in regulating the mating pheromone response in S. cerevisiae. EMBO rep. 2002 (3) 1215-1221


20. Maytal-Kivity V., Pick E., Piran R., Glickman M.H., The COP9 signalosome-like complex in S. cerevisiae and links to other PCI complexes, Int. J. Biochem. and Cell Biol., 2003 (35) 706-715


21. Bajorek M., Finley D., Glickman M.H., Proteasome Disassembly and Down regulation is Correlated with Viability during Stationary Phase, Current Biology, 2003 (13) 1140-1144


22. Guterman A., Glickman M.H., Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome, J. Biol. Chem., 2004, (279), 1729-1738.


23. Rinaldi T., Pick E., Gambadoro A., Zilli S., Maytal-Kivity V., Frontali L., Glickman M.H., Participation of Rpn11 in mitochondrial structure and function is mapped to a distinct C-terminal domain. Biochem. J. 2004 (380) 1-11.


24. Hetfeld, B.K, Helfrich, A., Kapelari, B., Scheel, H., Hofmann, K., Guterman, A., Glickman M.H, Schade R., Kloetzel P.M, Dubiel W., The Zinc Finger of the CSN-Associated Deubiquitinating Enzyme USP15 Is Essential to Rescue the E3 Ligase Rbx1. Current Biology 2005, (15) 1217-1221.


25. Rabinovich E., Bajorek M. Glickman M., Barnun S., "Proteasome Channel Opening as a Rate-Limiting Step in the Ubiquitin-Proteasome System", I. J. Chem., 2006, (46:2) 219-224.


26. Rabinovich E., Bussi D., Shapira I., Alalouf G., Lipson C., Elkabetz Y., Glickman M.H, Bajorek M., Bar-Nun S., "Quality control in the secretory pathway". Isr Med Assoc J. 2006 (8) 238-242.


27. Kleijnen M.F., Roelofs J., Park S., Hathaway N.A., Glickman M., King R.W., Finley D.Stability of the proteasome can be regulated allosterically through engagement of its proteolytic active sites. Nature Struct. Mol. Biol. 2007 (14) 1180-1188.


28. Porat Z., Landau G., Bercovich Z., Krutauz D., Glickman M., Kahana C. Yeast antizyme mediates degradation of yeast ornithine decarboxylase by yeast but not by mammalian proteasome: New insights on yeast antizyme J. Biol. Chem., 2008 (283) 4528-4534


29. Lipson C., Alalouf G., Bajorek M., Glickman M.H., Barnun S., "A Proteasome ATPase contributes to dislocation of ERAD substrates" J. Biol. Chem. 2008 (283) 7166-7175


30. Rinaldi T., Hofmann L., Gambadoro A., Cossard R., Livnat-Levanon N, Glickman M.H., Frontali L, and Delahodde A, Dissection of the Carboxyl-Terminal Domain of the Proteasomal Subunit Rpn11 in Maintenance of Mitochondrial Structure and Function, Mol. Biol. Cell, 2008 (19) 1022-1031


31. Cohen M., Leboucher G., Livnat-Levanon N., Glickman M.H.*, Weissman A.M.* (*corresponding), "Ubiquitin-Proteasome Dependent Degradation of a Mitofusin, a Critical Regulator of Mitochondrial Fusion". Mol. Biol. Cell, 2008 (19), 2457-2464. Featured article


32. Matiuhin Y., Kirkpatrick D.S., Ziv I., Kim W., Dakshinamurthy A., Kleifeld O., Gygi S.P., Reis N., Glickman M.H. Extraproteasomal Rpn10 restricts access of the polyubiquitin-binding protein Dsk2 to proteasome, Mol. Cell 2008 (32), 415-425 featured article


33. Rosenzweig R., Osmulski P.A., Gaczynska M., Glickman M.H., "The Central unit of the proteasome 19S regulatory particle" Nature Struct. and Mol. Biol. 2008 (15) 573-580.


34. Chen, T., Zhang, D., Matiuhin, Y., Glickman, M. and Fushman, D. 1H, 13C, and 15N resonance assignment of the ubiquitin-like domain from Dsk2p. Biomolecular NMR Assignments, 2008 (2) 147-149.


35. Effantin, G., Rosenzweig, R., Glickman, M.H. and Steven, A.C. Electron Microscopic Evidence in Support of [alpha]-Solenoid Models of Proteasomal Subunits Rpn1 and Rpn2. Journal of Molecular Biology, 2009 (386), 1204-1211.


36. Zhang D., Chen T., Ziv I., Rosenzweig R., Matiuhin Y., Bronner V., Lee S., Glickman M.H*, and Fushman D* (* corresponding authors). Together Rpn10 and Dsk2 serve as a polyubiquitin chain-length sensor. Mol. Cell, 2009 (36) 1018-1033.


37. Sha Z., Brill L.M., Kleifeld O., Glickman M.H., Chang E.C., Wolf D.A., The eIF3 Interactome Reveals the Translasome, a Supercomplex Linking Protein Synthesis and Degradation Machineries.Mol Cell. 2009 Oct 9;36(1):141-52


38. Heo JM, Livnat-Levanon N, Taylor EB, Jones KT, Dephoure N, Ring J, Xie J, Brodsky JL, Madeo F, Gygi SP, Ashrafi K, Glickman MH, Rutter J. A stress-responsive system for mitochondrial protein degradation. Mol Cell. 2010 Nov 12;40(3):465-80.


39. Cohen MM, Amiott EA, Day AR, Leboucher GP, Pryce EN, Glickman MH, McCaffery JM, Shaw JM, Weissman AM., Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion. J Cell Sci. 2011 May 1;124(Pt 9):1403-10.


40. Yu Z, Kleifeld O, Lande-Atir A, Bsoul M, Kleiman M, Krutauz D, Book A, Vierstra RD, Hofmann K, Reis N, Glickman MH*, Pick E*, Dual function of Rpn5 in two PCI complexes, the 26S proteasome and COP9 signalosome, Mol Biol Cell. 2011 Apr;22(7):911-20. Epub 2011 Feb 2.


41. Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH*, Kleifeld O*, A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis., Mol Cell Proteomics. 2011 May;10(5):M111.009753. Epub 2011 Mar 22.


42. Savulescu AF, Shorer H, Kleifeld O, Cohen I, Gruber R, Glickman MH, Harel A., Nuclear import of an intact preassembled proteasome particle., Mol Biol Cell. 2011 Mar;22(6):880-91. Epub 2011 Feb 2


43. Deriziotis P, André R, Smith DM, Goold R, Kinghorn KJ, Kristiansen M, Nathan JA, Rosenzweig R, Krutauz D, Glickman MH, Collinge J, Goldberg AL, Tabrizi SJ. Misfolded PrP impairs the UPS by interaction with the 20S proteasome and inhibition of substrate entry.EMBO J. 2011 Jul 8;30(15):3065-77.


44. Keren-Kaplan T, Attali I, Motamedchaboki K, Davis BA, Tanner N, Reshef Y, Laudon E, Kolot M, Levin-Kravets O, Kleifeld O, Glickman M, Horazdovsky BF, Wolf DA, Prag G, Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria, EMBO J. 2011 Nov 11. doi: 10.1038


45. He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP. The structure of the 26S proteasome subunit Rpn2 reveals a novel fold with a toroidal PC repeat domain, Structure 2012 Mar 7;20(3):513-521


46. Rosenzweig R, Bronner V, Zhang D, Fushman D, Glickman MH. Rpn1 and Rpn2 coordinate ubiquitin processing factors at the proteasome J Biol Chem. 2012 Apr 27;287(18):14659-71.


47. Leboucher GP, Tsai YC, Yang M, Shaw KC, Zhou M, Veenstra TD, Glickman MH, Weissman AM, Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis. Mol Cell. 2012 Aug 24;47(4):547-57. doi: 10.1016/j.molcel.2012.05.041. Epub 2012 Jun 28


48. Singh RK, Zerath S, Kleifeld O, Scheffner M, Glickman MH*, Fushman D*, Recognition and Cleavage of Rub1 and Rub1-Ubiquitin Chains by Components of the Ubiquitin-Proteasome System. Mol Cell Proteomics. 2012 Oct 26. [Epub ahead of print]


49. Bagola K, von Delbrück M, Dittmar G, Scheffner M, Ziv I, Glickman MH, Ciechanover A, Sommer T. Ubiquitin Binding by a CUE Domain Regulates Ubiquitin Chain Formation by ERAD E3 Ligases. Mol Cell. 2013 May 23;50(4):528-39. doi: 10.1016/j.molcel.2013.04.005. Epub 2013 May 9.


50. Nakasone MA, Livnat-Levanon N, Glickman MH, Cohen RE, Fushman D. Mixed-linkage ubiquitin chains send mixed messages. Structure. 2013 May 7;21(5):727-40. doi: 10.1016/j.str.2013.02.019. Epub 2013 Apr 4.


51. Livnat-Levanon Nurit, Kleifeld Oded, Kevei Éva, Rinaldi Theresa, Krutauz Daria, Erpapazoglou Zoi, Cohen Mickael, Reis Noa, Hoppe Thorsten, and Glickman Michael H, 26S Proteasome disassembly as a response to mitochondria dysfunction or oxidative stress (Manuscript under revision at Cell Metabolism).



REVIEW PAPERS and Book Chapters

R1. Glickman, M.H., Klinman, J.P., Isotopic probes of the Soybean Lipoxygenase-1 mechanism, in Lipoxygenase and Lipoxygenase Pathway Enzymes, George J. Piazza (ed.), AOCS Press, 1996.


R2. Finley, D., Tanaka, K., Glickman, M.H., et. al. Unified Nomenclature for subunits of the S. cerevisiae proteasome regulatory particle. Trends Biochem. Sci. 1998 (271) 244-245.


R3. Glickman, M.H., Rubin, D.M., Larsen, C.N., Finley, D. The regulatory particle of the yeast proteasome in "Proteasomes” Eds. W. Hilt and D. Wolf. 2000, Springer-Verlag, Germany.


R4. Deng, X.W., Dubiel, W., Wei, N., Hofmann, K., Mundt, K., Colicelli, J., Kato, J.Y., Nauman, M., Segal, D., Seeger, M., Glickman, M.H., Chamovitz, D.A. Unified nomenclature for the COP9 signalosome: an essential regulator of development. Trends Genet. Sci. 2000 (16) 202-203.


R5. Glickman, M.H. Getting in and out of the proteasome, Seminars in Cell Dev. Biol. 2000 (11) 149-158


R6. Glickman, M.H., Coux, O., Purification and characterization of proteasomes from Saccharomyces cerevisiae in "Current Protocols in Protein Science”, 2001, Chapter. 24, John Wiley & Sons, New York. 21.5.1-21.5.17


R7. Glickman, M.H., Ciechanover, A. The Ubiquitin-proteasome Proteolytic Pathway: Destruction for the sake of construction, Physiological Reviews 2002 (82) 373-428.


R8. Glickman, M.H., Maytal, V., Regulating the 26S proteasome, Curr. Top. Microbiol. and Immunol. 2002 (268) 42-72.


R9. Chamovitz, D., Glickman, M.H., The COP9 signalosome, Curr. Biol. 2002 (12) R232.


R10. Ciechanover, A., Glickman, M.H., The Ubiquitin-Mediated Proteolytic System, in "Life Sciences for the 21st century”, eds. Keinan, U., Shechter, I., Sela, M., VCH-Wiley Press 2003.


R11. Legget, D., Glickman, MH., Finley, D., "Purification of proteasomes, proteasome subcomplexes and proteasome-associated proteins from budding yeast” in  Ubiquitin-Proteasome Protocols eds. Patterson C and Cyr D. Methods in Molecular Biology, Humana Press 2005, Vol 301:57-70


R12. Ciechanover A. and Glickman, M.H., The ubiquitin pathway. The Encyclopedia of Biological Chemistry, eds. Lennarz WJ and Lane MD, Academic Press/Elsevier Science, volume 4 (2004)


R13. Guterman, A., Glickman, M.H., Deubiquitinating Enzymes are IN(trinsic to Proteasome function). Curr. Prot. Pept. Sci. 2004 (5) 201-210.


R14. Bajorek, M., Glickman, M.H., Keepers at the final gates: regulatory complexes and gating of the proteasome channel. Cell. Mol. Life Sci. 2004 61(13):1579-88


R15. Glickman, M.H. and Adir, N., The proteasome and the delicate balance between destruction and rescue, PLoS Biology 2004 (2) E13:25-27


R16. Bajorek, M. and Glickman, M.H., The proteasome portal and regulation of proteolysis, in Protein degradation Vol II. eds. Mayer R, Ciechanover A and Rechsteiner M, Wiley-VCH Press 2005.


R17. Glickman MH and Ciechanover A., The ubiquitin-proteasome system for controlling cellular protein levels, in The Encyclopedia of Molecular Cell Biology and Molecular Medicine, ed. Robert Mayer, WILEY-VCH Verlag 2005


R18. Lederkremer, G., Glickman, M.H., "A window of opportunity: Timing protein degradation by trimming of sugars and ubiquitins", TIBS, 2005, 30:297-303


R19. Glickman M.H., Raveh D. "Proteasome Plasticity" FEBS Lett. 2005 579(15):3214-23.


R20. Matiuhin Y., Glickman M.H., Ubiquitin and Ubiquitination, in The Ubiquitin Proteasome System In Central Nervous System: From Physiology To Pathology,  eds. Di Napoli M, Keller J.N.; Wojcik C., NovaScience Publisher Inc 2007 ch. 3, p 41-70.


R21. Glickman MH, Keinan E., "Ubiquitous Ubiquitin", IJC 2006 46(2):i-iii


R22. Rosenzweig, R. & Glickman, M. H. "Forging a proteasome [alpha]-ring with dedicated proteasome chaperones". Nature Struct. Mol. Biol. 2008 (15) 218-220.


R23. Rosenzweig R, Glickman MH. Chaperone-driven proteasome assembly. Biochem Soc Trans.2008 36(Pt 5) 807-812.


R24. Ziv I, Kleifeld O, Glickman MH, "Nonconformity in Ubiquitin Compliance" EMBO J 2009, 8;28(13):1825-1827


R25. Pick E. Hofmann K, Glickman MH "PCI complexes beyond the troika of Proteasome, CSN and eIF3" Mol. Cell 2009, 14;35(3):260-264).


R27. Livnat-Levanon N, Glickman MH, Ubiquitin-proteasome system and mitochondria - reciprocity., Biochim Biophys Acta. 2011 Feb;1809(2):80-87


R28. Savulescu AF, Glickman MH., Proteasome Activator 200: The HEAT is on..., Mol Cell Proteomics. 2011 May;10(5):R110.006890.


R29. Livnat-Levanon N, Glickman MH, The Poh1/Rpn11 peptidase for the Handbook of Proteolytic Enzymes MS.NO 361| (In press 2011)


R30. Bar-Nun S, Glickman MH. Proteasomal AAA-ATPases: Structure and function.Biochim Biophys Acta. 2012 1823(1):67-82