BEGIN:VCALENDAR VERSION:2.0 PRODID:-//wp-events-plugin.com//7.2.2.1//EN TZID:Asia/Jerusalem X-WR-TIMEZONE:Asia/Jerusalem BEGIN:VEVENT UID:1297@biology.technion.ac.il DTSTART;TZID=Asia/Jerusalem:20250715T130000 DTEND;TZID=Asia/Jerusalem:20250715T133000 DTSTAMP:20250701T085506Z URL:https://biology.technion.ac.il/en/seminars/7604/ SUMMARY:Msc Graduate Seminar-Tamar Gil [No Categories] DESCRIPTION:Location: Place: Hybrid - in the Faculty Auditorium /ZOOM: http s://technion.zoom.us/j/97752400169 \n Affiliation: \n Host:Prof. Roy Kish ony\n High-throughput mapping of transposon-driven genomic rearrangements under antibiotic stress\n\n \;\n\nTransposons and insertion sequence ( IS elements) facilitate bacterial adaptation to antibiotics through two ma in mechanisms. In regular transposition (insertion)\, an IS is excised fro m the genome and inserted at a target site. In replicative transposition\, each side of the IS attaches to another genomic locus\, joining them toge ther as it duplicates. These transpositions generate genomic rearrangement s and repeats\, which cannot be resolved by short reads. To overcome this challenge\, we couple inverse PCR with paired-end sequencing\, to jointly capture both IS flanks. Joint sequencing is achieved by fragmenting genomi c DNA\, self-ligating the fragments\, and PCR with outward-facing primers to target the now-fused flanks of the IS. We apply this method to IS1 in E . coli populations exposed to antibiotics\, and identify over 100 insertio n sites\, and a variety of locus-joining events. Comparison with control p opulations suggest adaptive amplification events via homologous recombinat ion between ISs\, as well as replicative transpositions. LOCATION:Place: Hybrid - in the Faculty Auditorium /ZOOM: https://technion. zoom.us/j/97752400169 END:VEVENT BEGIN:VTIMEZONE TZID:Asia/Jerusalem X-LIC-LOCATION:Asia/Jerusalem BEGIN:DAYLIGHT DTSTART:20250328T030000 TZOFFSETFROM:+0200 TZOFFSETTO:+0300 TZNAME:IDT END:DAYLIGHT END:VTIMEZONE END:VCALENDAR