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UID:587@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20150816T100000

DTEND;TZID=Asia/Jerusalem:20150816T110000

DTSTAMP:20210802T125839Z

URL:https://biology.technion.ac.il/en/seminars/dr-ori-avinoam-post-doctora
 l-fellow-embl-heidelberg-2/

SUMMARY:Dr. Ori Avinoam Post Doctoral fellow EMBL\, Heidelberg [No Categori
 es]
DESCRIPTION:Location:   \n Affiliation: \n Host:\n Clathrin-coated pits mat
 ure by continuous bending and remodeling of the coat\n\nAbstract\n\nClathr
 in mediated endocytosis (CME) is a fundamental eukaryotic process playing 
 essential roles in nutrient uptake\, membrane recycling\, synaptic transmi
 ssion and viral infection. Clathrin coated pits were among the first cellu
 lar structures described by electron microscopy over 5 decades ago\, and h
 ave been intensively studied ever since. Nevertheless\, researchers remain
  divided between two contradictory models for how clathrin coated vesicles
  are formed. Clathrin coated vesicles (CCVs) may form either through bendi
 ng of a pre-assembled flat coat\, or through coat assembly around the emer
 ging vesicle directly. To elucidate the mechanism of CCV formation\, I hav
 e applied two imaging approaches – high precision correlative light and 
 electron tomography\, and targeted fluorescence recovery after photobleach
 ing (FRAP). I found that clathrin assembles into a defined flat lattice ea
 rly in endocytosis\, predetermining the size of the vesicle\, and then rea
 rranges through dynamic exchange with the cytosolic pool to wrap around th
 e forming vesicle. This finding resolves the long-standing conflict about 
 the mechanism of CME\, leading perhaps to a surprising understanding of en
 docytosis where clathrin’s role in defining the endocytic site is indepe
 ndent of its characteristic assembly into curved polygonal shells\, and is
  mediated by dynamic instability rather than stable polymerization. Furthe
 rmore\, this study provides the first detailed quantitative description of
  membrane shape changes during endocytosis\, data which will be essential 
 for biophysical modeling. 

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