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UID:1341@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20251217T130000

DTEND;TZID=Asia/Jerusalem:20251217T140000

DTSTAMP:20251215T125531Z

URL:https://biology.technion.ac.il/en/seminars/faculty-seminar-jenia-binen
 baum-crispr-targeting-of-h3k4me3-reprograms-transcription-immunity-and-mei
 otic-recombination-in-plants/

SUMMARY:Faculty Seminar- Jenia Binenbaum  "CRISPR targeting of H3K4me3 Repr
 ograms Transcription\, Immunity\, and Meiotic Recombination in Plants". [N
 o Categories]
DESCRIPTION:Location: Faculty Of Biology Auditorium  Jenia Binenbaum \n Aff
 iliation: University of Cambridge\, United Kingdom)\n Host:Dr\, Maya Maor-
 Nof \n CRISPR targeting of H3K4me3 Reprograms Transcription\, Immunity\, a
 nd Meiotic \nRecombination in Plants\nPlants can develop a form of molecul
 ar memory through repeated exposure to specific stresses\,\nallowing them 
 to respond more effectively upon subsequent challenges\, a process known a
 s\npriming. Primed genes have an enhanced transcriptional responsiveness u
 pon repeated exposure\nto stress\, while returning to baseline levels of e
 xpression in the absence of stimuli. These genes\nare thought to remain in
  ‘primed’ or ‘poised’ state\, with an altered chromatin landscape 
 that\nfacilitates hyper-induction upon a recurrent stress event. We aim to
  synthetically recreate the\npathogen-primed state in Arabidopsis thaliana
  by targeted deposition of H3K4me3. To achieve\nthis\, we employ the CRISP
 R-based SunTag system to recruit the catalytic domain of SDG2\, a key\nhis
 tone methyltransferase responsible for H3K4me3 deposition in plants\, to t
 argeted promoters.\nOur work shows that H3K4me3 deposition by SunTag-SDG2 
 is sufficient to transcriptionally\nactivate the epigenetically silenced g
 ene FWA\, establishing that H3K4me3 itself can act as a causal\nregulatory
  mark. Next we demonstrate that SunTag-SDG2 can be employed to increase pa
 thogen\nresistance by targeting the H3K4me3-regulated disease resistance g
 ene\, SNC1. Extending this\napproach to pathogen memory\, we show that Sun
 Tag-directed H3K4me3 deposition can\nsuccessfully prime a WRKY-family tran
 scription factor\, further enhancing pathogen resistance.\nBeyond transcri
 ptional regulation\, targeting SunTag-SDG2 to low recombining centromeric\
 nregions significantly increases proximal crossover formation. Together\, 
 these findings establish\nthat locus-specific H3K4me3 deposition is able t
 o modulate transcription\, immunity\, stress\nmemory and recombination\, h
 ighlighting the potential of chromatin engineering as a tool for\nprecise 
 modulation of important agricultural traits 
LOCATION:Faculty Of Biology Auditorium

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