BEGIN:VCALENDAR VERSION:2.0 PRODID:-//wp-events-plugin.com//7.2.2.1//EN TZID:Asia/Jerusalem X-WR-TIMEZONE:Asia/Jerusalem BEGIN:VEVENT UID:1192@biology.technion.ac.il DTSTART;TZID=Asia/Jerusalem:20230829T133000 DTEND;TZID=Asia/Jerusalem:20230829T140000 DTSTAMP:20230822T093245Z URL:https://biology.technion.ac.il/en/seminars/msc-graduate-seminar-dolev- elisha/ SUMMARY:Msc Graduate Seminar- Dolev Elisha [No Categories] DESCRIPTION:Location: Dolev Elisha\n Affiliation: \n Host:Associate Prof . Henn Arnon \n Title:\n\nRole of Myo19\, an actin-based mitochondria moto r\, during collective cell migration.\n\n \n\nAbstract:\n\nCellular migra tion is essential for embryonic development\, wound healing\, immune defen se\, and angiogenesis\, but it also is an enhancer of cancer metastasis an d inflammation. Cell migration is described as cycles of continuous actin branching at the cell leading edge\, focal adhesion (FA) sites assembly\, and disassembly and contraction of the cell trailing end. These highly ene rgy-consuming processes require positioning the mitochondria at FA sites a nd the leading edge of cells to supply ATP. In eukaryotes\, mitochondria a re solely associated with actin and microtubule filaments. The actin-based transport of mitochondria is mainly by the molecular motor Myo19\, which has been shown to transport mitochondria to actin protrusions at the cell ’s leading edge during cellular stress. We hypothesize that Myo19 plays an essential role in the motility and positioning of mitochondria in the c ell’s leading edge during migration. My goal is to determine the contrib ution of Myo19 during cellular migration. I will generate stable cell line s of inducible KD of Myo19 and perform collective in vitro cell migration by automated high throughput scratch assay ('wound healing') on stable Myo 19 KD cells. To this end\, the results display a significant reduction in cell migration rates in several cell lines. This suggests that Myo19 plays a role in coupling mitochondria positioning and mobility during cellular migration. Further research on the regulation of Myo19 might provide a new approach to metastatic cancer therapy.\n\n \;\n\n \; END:VEVENT BEGIN:VTIMEZONE TZID:Asia/Jerusalem X-LIC-LOCATION:Asia/Jerusalem BEGIN:DAYLIGHT DTSTART:20230324T030000 TZOFFSETFROM:+0200 TZOFFSETTO:+0300 TZNAME:IDT END:DAYLIGHT END:VTIMEZONE END:VCALENDAR