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UID:1201@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20231227T130000

DTEND;TZID=Asia/Jerusalem:20231227T133000

DTSTAMP:20231221T082737Z

URL:https://biology.technion.ac.il/en/seminars/msc-graduate-seminar-galia-
 polyak/

SUMMARY:Msc Graduate Seminar- Galia Polyak [No Categories]
DESCRIPTION:Location: Hybrid- in the Faculty Auditorium/ZOOM: https://techn
 ion.zoom.us/j/9909936173?omn=94601376175    Galia Polyak\n Affiliation: \n
  Host:Prof. Yael Mandel-Gutfreund \n Combining RNA- and protein-centric ap
 proaches to discover new functions of non-coding RNAs during stem cell dif
 ferentiation\nNon-coding RNAs are at the interplay between transcription a
 nd post transcription regulation. Long non-coding RNAs\, in particular\, o
 ften exert their function by interacting with proteins. In this study\, we
  applied high throughput methodologies combining RNA-centric and protein-c
 entric approaches to identify RNA-protein interactions involved in transcr
 iption regulation in pluripotency.\nWe started by revealing the protein-bo
 und transcriptome of hESCs\, embryoid bodies\, and each germ layer. We ide
 ntified the lncRNAs that changed their binding without correlating changes
  in expression\, as well as those unique to pluripotency\, differentiated 
 cells\, or each germ layer specifically.\nWe chose the epigenetic factor D
 NMT3b to further study protein-RNA interactions in hESCs. We found it inte
 racts with the RNAs of 283 genes related to development and transcription\
 , and enriched in CpG islands. DNMT3b’s RNA targets were simultaneously 
 significantly more methylated at the DNA level than other expressed genes\
 , and more expressed. We also found that DNMT3b binds the same motifs at t
 he RNA and DNA level. These DNMT3b/RNA interactions could modulate DNMT3b
 ’s DNA binding and methylation to regulate the expression of key genes i
 n development.\n 
LOCATION:Hybrid- in the Faculty Auditorium/ZOOM: https://technion.zoom.us/j
 /9909936173?omn=94601376175 

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DTSTART:20231029T010000

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