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UID:1194@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20230912T130000

DTEND;TZID=Asia/Jerusalem:20230912T133000

DTSTAMP:20230823T121754Z

URL:https://biology.technion.ac.il/en/seminars/msc-graduate-seminar-hadas-
 gruber/

SUMMARY:Msc Graduate Seminar- Hadas Gruber [No Categories]
DESCRIPTION:Location: Faculty Of Biology Auditorium/ZOOM:https://technion.z
 oom.us/j/92857653725   Hadas Gruber\n Affiliation: \n Host: 	Prof. Melamed
  Philippa\n  \n\nFoxl2 and Nr5a1 regulation in gonadotrope cells of the a
 nterior pituitary\n\n&nbsp\;\n\nThe mammalian reproductive system is regul
 ated by gonadotrope cells in the anterior pituitary gland. These cells pro
 duce and secrete the gonadotropin hormones\, luteinizing hormone (LH) and 
 follicle stimulating hormone (FSH)\, responsible for germ cell maturation.
  Gonadotrope differentiation in the developing pituitary is driven by seve
 ral transcription factors (TFs)\, among them FOXL2 and SF-1 (encoded by FO
 XL2 and NR5A1\, respectively). FOXL2 and SF-1 also support mature gonadotr
 ope function\, notably expression of FSHB which encodes the FSH β-subunit
 . Despite their essential role in gonadotropes\, little is known about how
  these factors are regulated in the pituitary. In this research\, we aimed
  to find what regulates FOXL2 and NR5A1 in both differentiating and mature
  gonadotrope cells. We hypothesized that expression of both genes is activ
 ated by pituitary-specific factors and regulatory elements. We discovered\
 , surprisingly\, that activin A and GnRH\, which both stimulate Fshb expre
 ssion\, repress Foxl2 and Nr5a1 expression in murine gonadotrope cells. Bo
 th Foxl2 and Nr5a1 have adjacent sequences transcribed to divergent long n
 on-coding RNAs (lncRNAs)\, Foxl2os and Nr5a1os\, that are co-expressed in 
 a tissue-specific manner and respond to the same stimulus as the protein c
 oding genes. Of these lncRNAs\, Foxl2os shows potential as a Foxl2 regulat
 or\, possibly through the formation of G-quadruplexes and iMotifs that we 
 detected and appear to impact Foxl2os levels. Additionaly\, using publishe
 d ATAC-seq data\, we have identified a putative gonadotrope specific super
 -enhancer upstream of the Foxl2 promoter. Targeting dCas9-KRAB to this reg
 ion reduced Foxl2 mRNA levels\, indicating a functional role. This functio
 n is supported by the presence of annotated ChIP-seq binding of TFs that a
 re expressed in differentiating pre-gonadotropes prior to Foxl2. These res
 ults offer novel candidates for the regulation of two genes crucial for hu
 man fertility.\n\n&nbsp\;\n\nThe seminar will be given in English.\n\n  
LOCATION:Faculty Of Biology Auditorium/ZOOM:https://technion.zoom.us/j/9285
 7653725

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