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UID:965@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20210629T130000

DTEND;TZID=Asia/Jerusalem:20210629T140000

DTSTAMP:20210802T125905Z

URL:https://biology.technion.ac.il/en/seminars/msc-graduate-seminar-lama-a
 amar-2/

SUMMARY:MSc Graduate Seminar-Lama Aamar [No Categories]
DESCRIPTION:Location:   \n Affiliation: \n Host:\n  The development and gr
 owth of the plant body are regulated by aninterplay between plant hormone 
 signaling pathways that both interpret anddetermine their accumulation and
  distribution. Brassinosteroids (BRs) are oneof these hormones. Although t
 he BR signaling pathway is well known\, there arestill gaps in our knowled
 ge regarding the site of BR production\, the extent ofBR movement\, and ho
 w its signaling pathway is decoded at the tissue andcellular levels. Using
  the Arabidopsis root as a developmental system\, recentstudies showed tha
 t BR biosynthesis genes are expressed in different tissues\,suggesting tha
 t the BR hormone might move radially between tissues. Inaddition\, BR sign
 aling has been shown to have different developmental effectson the root me
 ristem\, depending on the tissue in which it is perceived\,suggesting that
  BR signaling is decoded in a tissue-specific manner. Thisthesis was condu
 cted with the following open questions in mind: do activehormones or their
  precursors move between tissues in the root\, istissue-specific BR signal
 ing essential for meristem development\, and howcellular BR activity can b
 e quantified in the growing root. To address these questions\, I applied 
 established approaches and alsoevaluated new strategies. These were design
 ed to cover the three main steps ofthe hormone action: i) biosynthesis\, i
 i) signaling input (i.e.\, receptoractivity)\, and iii) signaling output (
 i.e.\, regulation of gene expression). Forthe first part\, I took a tissue
 -specific-complementation approach for BRproduction to evaluate the extent
  of BR movement in the root meristem. This wasperformed alongside the char
 acterization of the root system architecture andits dependency on BR biosy
 nthesis. For studies involving BR signaling input\, Iapplied a novel appro
 ach for a tissue-specific knockout using the CRISPR-CAS9technology. This t
 ool aimed to target the BR receptor BRI1 in select tissues asa means to in
 fer the essentiality of BRI1’s activity in a particular tissue inthe mer
 istem. Finally\, I started to develop a spatiotemporal transcriptionalread
 out for BR signaling output as part of a collaborative effort. Thisstrateg
 y is based on designing a promoter sequence that includes conservedmotifs 
 from promoters activated as a response to BR presence. This promoter isfus
 ed to nuclear localization fragment and fluorophore to detect BR responses
 ites in the meristem different tissues of the root. The results of theseap
 proaches will be presented and discussed. 

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