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UID:1242@biology.technion.ac.il

DTSTART;TZID=Asia/Jerusalem:20240910T133000

DTEND;TZID=Asia/Jerusalem:20240910T140000

DTSTAMP:20240828T081259Z

URL:https://biology.technion.ac.il/en/seminars/msc-graduate-seminar-shiran
 -rachel-peretz/

SUMMARY:MSc Graduate Seminar-Shiran Rachel Peretz [No Categories]
DESCRIPTION:Location: Hybrid- in the Faculty Auditorium/ZOOM: https://techn
 ion.zoom.us/j/92090707928    Shiran Rachel Peretz\n Affiliation: \n Host:D
 r. Nadav Sharon  \n In search of pancreatic beta cells with a regenerative
  potential\n\nShiran Rachel Peretz\n\n&nbsp\;\n\nDepletion of β cell mass
  is a key characteristic of diabetes\, impairing insulin production and gl
 ucose regulation\, which sparks interest in restoring beta cell mass throu
 gh regeneration. While β cells rarely divide\, their expansion under spec
 ific conditions such as high-fat diet\, pregnancy\, or pancreatic injury s
 uggests that regulating mature β cell proliferation is possible. A key qu
 estion is whether all beta cells can proliferate or\, alternatively\, cert
 ain beta cell subsets have higher proliferative capacity.\n\nCharacterizin
 g dormant stem cell sub-populations is methodologically challenging becaus
 e the only way to determine if a cell has the potential to divide is to ob
 serve it proliferate\, but post-division\, its pre-proliferative character
 s may be lost and no longer observable.\n\nTo overcome the limitations of 
 conditions that induce β cell proliferation\, we use an insulin receptor 
 antagonist (S961)\, which acutely elevates blood glucose levels and mimics
  the type 2 diabetes state\, characterized by β cell proliferation.\n\nTo
  establish whether all beta cells can divide or not\, we adapted a CRISPR-
 based lineage tracing model (CARLIN) to become β cell specific\, enabling
  cell-specific genetic barcoding\, followed by triggering of beta cell rep
 lication. Disproportionate proliferation should lead to non-uniform barcod
 e distribution\, and thereby answer the question unequivocally.\n\nTo char
 acterize the proliferative cells further\, we combine S961-inducible repli
 cation with a tamoxifen-induced Ki67 Cre/loxp-TdTomato mouse model (M/T)\,
  which permanently labels dividing β cells and facilitates tracking them 
 over time. Our results support the existence of a subpopulation of prolife
 rative β cells\, which is present in most islets.\n\n&nbsp\; 
LOCATION:Hybrid- in the Faculty Auditorium/ZOOM: https://technion.zoom.us/j
 /92090707928  

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