BEGIN:VCALENDAR VERSION:2.0 PRODID:-//wp-events-plugin.com//7.2.2.1//EN TZID:Asia/Jerusalem X-WR-TIMEZONE:Asia/Jerusalem BEGIN:VEVENT UID:1171@biology.technion.ac.il DTSTART;TZID=Asia/Jerusalem:20220802T130000 DTEND;TZID=Asia/Jerusalem:20220802T140000 DTSTAMP:20230424T094239Z URL:https://biology.technion.ac.il/en/seminars/phd-graduate-seminar-jessy- safieh-2/ SUMMARY:PhD Graduate Seminar- Jessy Safieh [No Categories] DESCRIPTION:Location: Faculty Of Biology Auditorium \n Affiliation: \n Hos t:Associate Prof. Haran Tali \n  \;\n\nOne of the master transcription al regulation hubs of every human cell is the transcription factor (TF) p5 3 protein. p53 is activated in response to cellular stress\, and is involv ed in the regulation of many cellular outcomes. To initiate transcription of downstream genes\, p53 binds in a sequence-specific manner to many site s on the genome\, called response elements (REs). Even after almost 40 yea rs of intensive research in the field of p53\, the fundamental question co ncerning how p53 activates its p53-dependent genes in response to the seve rity of the stress signal and consequently coordinates the functional outc ome in a timely manner is still poorly understood.\n\n \;\n\nIn this r esearch\, we showed that p53-dependent functional outcome groups can be di fferentiated by their RE flexibility. p53-dependent genes belonging to pat hways activated early upon stress (e.g. DNA damage response) contain REs t hat are more flexible than REs of genes involved in pathways that are like ly to be more strictly regulated\, or that should occur at late stages in the response to stress (e.g. intrinsic apoptosis\, p53 negative regulation ). Moreover\, based on data of chosen REs\, genes that are part of outcome categories in which a response is needed immediately\, timewise\, have RE s that are capable of transactivating the genes at a faster rate and at lo w levels of p53\, because they are flexible. Thus\, the flexibility of p53 RE contributes to the time-wise expression of p53 target genes and thereb y plays a key role in cell-faith decisions in the p53 circuity.\n\n \; \n\nIn addition\, we have indications\, using SELEX-seq technique\, that t he mechanistic source of selectivity in p53/REs interactions is encoded in the kinetic of p53/REs interactions\, rather than in the thermodynamics o f the interaction. LOCATION:Faculty Of Biology Auditorium END:VEVENT BEGIN:VTIMEZONE TZID:Asia/Jerusalem X-LIC-LOCATION:Asia/Jerusalem BEGIN:DAYLIGHT DTSTART:20220325T030000 TZOFFSETFROM:+0200 TZOFFSETTO:+0300 TZNAME:IDT END:DAYLIGHT END:VTIMEZONE END:VCALENDAR