“Computational approach to identify new roles for long non-coding RNAs involved in pluripotency and differentiation”
Recently long non coding RNAs (lncRNAs) have emerged as regulators of pluripotency and differentiation, however their contribution to specific lineage commitment is yet largely unknown. We generated RNA-seq data, in human induced Pluripotent Stem cells (iPSc) and applied a network approach to help assign lncRNAs with unknown functions to these processes. We found many differentially expressed lncRNAs highly co-expressed with known markers of differentiation. These results were validated with ChiP-seq data suggesting a possible regulatory relationship between the TFs and the lncRNAs.