Title: “Using various single-molecular assays to measure the passage of RNA polymerase along the DNA template in optical tweezers”
Abstract:
Transcription is the subsequent step in the gene expression process. that started with the binding of RNA polymerases (RNAP) a DNA template and transcribing it, leading to the release of an RNA molecule from the DNA template. Traditional “bulk” approaches are challenging to detect the dynamic nature of the transcription elongation in real-time. Optical tweezers offer a unique ability to address this problem. In my project, I sought to detect and characterize the RNAP movement utilizing and optimizing several approaches in optical tweezers based on the literatures and our experimental conditions.
