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Oded Kleifeld

Oded Kleifeld

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Education/ Resume:

Ph.D. Weizmann Institute 2004
M. Sc. Biochemistry, Tel-Aviv University 1998
B.Sc. Biology. Tel-Aviv University 1995

Research Summary

Our laboratory is interested in discovering the functional roles of proteases in different cellular processes and disease conditions. Our research combines biochemistry, cellular, and molecular biology with proteomics. We are developing various mass-spectrometry-based proteomic methods to monitor proteases and their activity in complex biological samples including cells, tissues, and whole organisms.

We are applying these tools to:

  • Investigations of the peroxisomal Lon protease homolog 2 (LonP2) and its role in maintaining the peroxisome homeostasis under stress conditions
  • Identification of novel substrates of proteases from the caspase and calpain families
  • Study the human and microbiome proteases in Inflammatory bowel diseases
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Key Publications
  • Overall, C. M. & Kleifeld, O. Validating matrix metalloproteinases as drug targets and anti-targets for cancer therapy. Nature Reviews Cancer (2006) doi:10.1038/nrc1821.
  • Kleifeld, O., Doucet, A., auf dem Keller, U., Prudova, A., Schilling, O., Kainthan, R. K., Starr, A. E., Foster, L. J., Kizhakkedathu, J. N. & Overall, C. M. Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products. Nature Biotechnology 28, 281–8 (2010).
  • Ziv, I., Matiuhin, Y., Kirkpatrick, D. S., Erpapazoglou, Z., Leon, S., Pantazopoulou, M., Kim, W., Gygi, S. P., Haguenauer-Tsapis, R., Reis, N., Glickman, M. H.* & Kleifeld, O.* A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis. Molecular and Cellular Proteomics (2011) doi:10.1074/mcp.M111.009753.

* Equally contributed last and corresponding Author

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Recent Publications:
  • Sahu, I., Mali, S.M., Sulkshane,P., Rozenberg, A., Xu, C., Morag, R., Sahoo, M.P., Singh, S.K., Ding, Z., Wang, Y., Day, S., Cong, Y. Kleifeld, O*., Brik, O.* & Glickman, M.H* Signature activities of 20S proteasome include degradation of the ubiquitin-tag with the protein under hypoxia BioRxiv 2019.12.20.883942
  • Silva, L. M., Kryza, T., Stoll, T., Hoogland, C., Dong, Y., Stephens, C. R., Hastie, M. L., Magdolen, V., Kleifeld, O., Gorman, J. J. & Clements, J. A. Integration of Two In-depth Quantitative Proteomics Approaches Determines the Kallikrein-related Peptidase 7 (KLK7) Degradome in Ovarian Cancer Cell Secretome. Molecular and Cellular Proteomics 18, (2019).
  • Meledin, R., Mali, S. M., Kleifeld, O.* & Brik, A.* Activity-Based Probes Developed by Applying a Sequential Dehydroalanine Formation Strategy to Expressed Proteins Reveal a Potential α-Globin-Modulating Deubiquitinase. Angewandte Chemie – International Edition 57, 5645–5649 (2018).
  • Silva, L. M., Kryza, T., Stoll, T., Hoogland, C., Dong, Y., Stephens, C. R., Hastie, M. L., Magdolen, V., Kleifeld, O., Gorman, J. J. & Clements, J. A. Integration of Two In-depth Quantitative Proteomics Approaches Determines the Kallikrein-related Peptidase 7 (KLK7) Degradome in Ovarian Cancer Cell Secretome. Molecular and Cellular Proteomics 18, (2019).
  • Meledin, R., Mali, S. M., Kleifeld, O.* & Brik, A.* Activity-Based Probes Developed by Applying a Sequential Dehydroalanine Formation Strategy to Expressed Proteins Reveal a Potential α-Globin-Modulating Deubiquitinase. Angewandte Chemie – International Edition 57, 5645–5649 (2018).

* Equally contributed last and corresponding Author

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