Characterizing A-G Mismatch Signatures Associated with A-to-I RNA Editing and m6A in C. elegans
RNA molecules can undergo chemical modifications that influence how they are processed and regulated. Using Caenorhabditis elegans as a model system, we analyze RNA sequencing datasets to investigate different types of RNA modifications. Focusing on A-to-I RNA editing, we identify candidate editing sites in mature and precursor microRNAs and examine their expression dynamics during embryonic development. RNA sequencing data frequently contain nucleotide mismatches relative to the reference genome; while some can be explained by A-to-I RNA editing, many remain unexplained. We therefore analyze transcriptome-wide data to explore whether characteristic mismatch patterns in mRNA reads may reflect signatures associated with m6A modification. Together, these analyses provide insight into how RNA modifications shape RNA regulation and suggest potential avoidance between different RNA modification pathways.athways.
